s protein omicron variant  (BPS Bioscience)

Journal: Nature Communications

Article Title: Waning and boosting of antibody Fc-effector functions upon SARS-CoV-2 vaccination

doi: 10.1038/s41467-023-39189-8

Figure Lengend Snippet: A Scheme for vaccination series and subsequent systems serology analysis. Participants who received two doses of the inactivated vaccine CoronaVac were boosted with the mRNA vaccine BNT162b2. As controls, the two-dose mRNA vaccine recipients were also analyzed. Sera were collected at various time points and systems serology assays were conducted (see Table for specific days of sample collections). B Spike- (left) and receptor binding domain (RBD) (right) specific IgG1 levels for SARS-CoV-2 WT and early VOCs (Alpha and Beta) were quantified in the baseline (prior to immunization, white, lane 1), 1- and 2-dose BNT162b2 mRNA (blue, lanes 2 and 3, waning periods in lanes 4 and 5), 1- and 2-dose CoronaVac (gray, lanes 6 and 7, waning periods in lanes 8 and 9) via Luminex systems serology. Y -axis represents the MFI of binding in arbitrary units (A.U.) of a specific antigen. Shown are box and whiskers, along with individual data points, which represent the mean of individual participants from each vaccine group (BNT162b2, n = 15 and CoronaVac, n = 34). The whisker above the box plot extends from the top quartile to the highest actual value that is within the 75th percentile + 1.5 * interquartile range. The whisker below the box plot extends from the lower quartile to the lowest actual value that is within 75 th percentile + 1.5 * interquartile range. C Same as ( B ), but for latter VOC (Gamma, Delta, and Omicron) of SARS-CoV-2 VOC. All samples were assayed in technical duplicates. Kruskal–Wallis test was used for all panels (* p < 0.05 and ** p < 0.01). All Kruskal–Wallis tests were two-sided, and no adjustments were made for multiple comparisons. See also Supplementary Fig. . Source data are provided as a Source Data file.

Article Snippet: SARS-CoV-2 Omicron Variant S , Sino Biological , 40589-V08H26.

Techniques: Binding Assay, Luminex, Whisker Assay

Journal: Nature Communications

Article Title: Waning and boosting of antibody Fc-effector functions upon SARS-CoV-2 vaccination

doi: 10.1038/s41467-023-39189-8

Figure Lengend Snippet: A – D FcR antibody binding towards WT SARS-CoV-2 Spike was quantified in the baseline (prior to immunization, white, lane 1), 1- and 2-dose BNT162b2 mRNA (blue, lanes 2 and 3, waning periods in lanes 4 and 5), 1- and 2-dose CoronaVac (gray, lanes 6 and 7, waning periods in lanes 8 and 9) via Luminex systems serology. Y-axis represents the MFI of binding full-length WT SARS-CoV-2 Spike. Shown are box and whiskers, along with individual data points, which represent the mean of individual participants from each vaccine group (BNT162b2, n = 15 and CoronaVac, n = 34). The whisker above the box plot extends from the top quartile to the highest actual value that is within the 75th percentile + 1.5 * interquartile range. The whisker below the box plot extends from the lower quartile to the lowest actual value that is within 25th percentile + 1.5 * interquartile range. ( A ) FcγRIIA, ( B ) FcγRIIB, ( C ) FcγRIIIA, and ( D ) FcγRIIIB. E – H Same as ( A – D) , but for SARS-CoV-2 RBD. Note that Y -axis scales are not the same. All samples were assayed in technical duplicates. Kruskal-Wallis test was used for all panels (* p < 0.05 and ** p < 0.01). All Kruskal–Wallis tests were two-sided, and no adjustments were made for multiple comparisons. The samples were assayed in technical duplicates. See also Supplementary Fig. for SARS-CoV-2 Nucleocapsid and control results. Source data are provided as a Source Data file.

Article Snippet: SARS-CoV-2 Omicron Variant S , Sino Biological , 40589-V08H26.

Techniques: Binding Assay, Luminex, Whisker Assay

Journal: Nature Communications

Article Title: Waning and boosting of antibody Fc-effector functions upon SARS-CoV-2 vaccination

doi: 10.1038/s41467-023-39189-8

Figure Lengend Snippet: A Spike-specific IgG1 levels were measured at peak immunogenicity following the two-dose CoronaVac vaccination series (lane 1), during waning periods (lanes 2 and 3), and after mRNA boost (lane 4) for SARS-CoV-2 VOC (color legend shown on right). The Y -axis represents the MFI of the Spike from the VOC. Shown are means (solid line) with SEM for each VOC in the corresponding color in the shaded region. B Same as ( A ), but for the receptor-binding domains (RBD) of SARS-CoV-2 VOC. C Heatmap representation of binding for full-length Spike by antibody-subclasses and -isotypes and Fcγ-receptor complexes. Shown on the left are the description of CoronaVac doses, the subsequent waning period, and mRNA-vaccine booster, and on the right are the SARS-CoV-2 VOC or WT. The scale bar is shown to the right of the heatmap and represents MFI over baseline values. The values in each region represent the mean of values from individual participants from each vaccine group (BNT162b2, n = 15 and CoronaVac, n = 34). D Same as ( C ), but for the RBD of WT SARS-CoV-2 and VOC. The samples were assayed in technical duplicates. See also Supplementary Fig. . Source data are provided as a Source Data file.

Article Snippet: SARS-CoV-2 Omicron Variant S , Sino Biological , 40589-V08H26.

Techniques: Binding Assay

Journal: Nature Communications

Article Title: Waning and boosting of antibody Fc-effector functions upon SARS-CoV-2 vaccination

doi: 10.1038/s41467-023-39189-8

Figure Lengend Snippet: A (Left) Antibody-dependent complement deposition (ADCD) activities measured in fluorescent arbitrary units (A.U.) against the WT SARS-CoV-2 Spike (left) and Omicron Spike were quantified in the baseline (prior to immunization, white, lane 1), 1- and 2-dose BNT162b2 mRNA (blue, lanes 2 and 3, waning periods in lanes 4 and 5), 1- and 2-dose CoronaVac (gray, lanes 6 and 7, waning periods in lanes 8 and 9) and after mRNA-vaccine booster (lane 10). B Same as ( A ), but for antibody-dependent neutrophil phagocytosis (ADNP) measured in phagocytic A.U. C Same as ( A ) but for antibody-dependent monocytic cellular phagocytosis (ADCP) measured in phagocytic A.U. D Same as ( A ) but for the Primary natural killer (NK) cells activities measured for percent expression of MIP1b (top) and CD107a (bottom). Shown are box and whiskers, along with individual data points, which represent the mean of individual participants from each vaccine group (BNT162b2, n = 15 and CoronaVac, n = 34). The whisker above the box plot extends from the top quartile to the highest actual value that is within the 75th percentile + 1.5 * interquartile range. The whisker below the box plot extends from the lower quartile to the lowest actual value that is within 75th percentile + 1.5 * interquartile range. Note that Y-axis scales are not the same. All samples were assayed in technical quadruplicates with a minimum of three independent donors. Kruskal-Wallis test was used for all panels (* p < 0.05 and ** p < 0.01). All Kruskal–Wallis tests were two-sided, and no adjustments were made for multiple comparisons. See also Supplementary Fig. . Source data are provided as a Source Data file.

Article Snippet: SARS-CoV-2 Omicron Variant S , Sino Biological , 40589-V08H26.

Techniques: Expressing, Whisker Assay

Journal: Nature Communications

Article Title: Waning and boosting of antibody Fc-effector functions upon SARS-CoV-2 vaccination

doi: 10.1038/s41467-023-39189-8

Figure Lengend Snippet: List of reagents and resources used in this study

Article Snippet: SARS-CoV-2 Omicron Variant S , Sino Biological , 40589-V08H26.

Techniques: Binding Assay, Variant Assay, Chromatography, Software, Luminex